![]() The results showed a significant increase (P≤0.05) in levels of calcium in blood plasma for fifth, sixth, seventh, eighth and ninth treatments. ![]() The experimental treatments were as follows: T1/ control diet without any additives, T2/ calcium source 50% limestone + 50% oyster shell size less than 1 mm + vitamin D 3 4000 IU kg -1, T3/ calcium source 50% limestone +50% oyster shell size 1-2 mm + vitamin D 3 4000 IU kg -1, T4/ calcium source 50% limestone + 50% oyster shell size 2-3 mm + vitamin D 3 4000 IU kg -1, T5/ calcium source 50% Limestone + 50% oyster shell size 3–5 mm + Vitamin D 3 4000 IU kg -1, T6/ Calcium source 50% limestone + 50% oyster shell size less than 1 mm + Vitamin D 3 8000 IU kg -1, T7 / Calcium source 50% limestone + 50% oyster shell 1-2 mm +Vitamin D 3 8000 IU kg -1, T8 Calcium source 50% limestone + 50% oyster shell 2-3 mm + Vitamin D 3 8000 IU kg -1, T9/ Calcium source 50% limestone +50% oyster shell size 3-5mm + Vitamin D 3 8000 IU kg -1. This study was conducted in the poultry farm belong to Department of Animal Production at College of Agriculture, University of Anbar, from to, to show the role of vitamin D3 and multi particle size of oyster shells to providing the needed calcium for laying hens in order to manufacture the eggshell throughout the hours of the day and its impact on shell quality and bone health.one hundred-eight Lohman Brown laying hens, 43 weeks of age, were distributed randomly to nine treatments, four replicates for each treatment and three hens for each replicate.
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